Hi-Plex

Hi-Plex Columns from Agilent Technologies

Choosing to use guard columns can help reduce operating expenses, by reducing the frequency of analytical column replacement. The guard column prevents damage caused by particulate matter and strongly adsorbed material. To maintain an adequate capacity for sample impurities, choose a guard column with an internal diameter similar to the column internal diameter. Ideally, the packing of the guard column should be the same as the analytical column so that the chromatography of the analytical column is not altered.

Agilent's Hi-Plex HPLC column provides reliable carbohydrate analysis with simple instrument requirements. Unlike Ion Chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated polystyrene gel impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58.